Abstract: In order to study the stability of the optimal extraction conditions of Shi-qian Tai tea polysaccharides and tea polysaccharide in different conditions, the single factor and orthogonal test were conducted. The results showed as follows: the best extraction conditions of Tai tea polysaccharides were 40% ethanol volume fraction, the ratio of material to liquid was 1颐55 (g/mL, W/V), the extracting time was 2.5 h, and the temperature was nearly 70益, thus the extraction rate in these conditions was 2.87%; In addition, a preliminary study on the stability of Shi-qian Tai tea polysaccharides was carried out, as the results showed that the polysaccharides of the tea had good stability as it was strongly influenced by the light and reductant-oxidant; Under the conditions of different metal ions, Cu2+ and Fe3+ had the greatest impact.