灭草烟在甘蔗愈伤分化阶段和刚成苗阶段浓度筛选试验

Isolation and identification of endophytic bacterial strain LYM3 from Rauvolfia vomitoria and its fermentation conditions optimization for production of antibacterial substance

摘要: 以甘蔗品种新台糖 22 和粤糖 93-159 的愈伤组织和刚分化 4 周的小苗为试验材料，初步确定了灭草烟在愈伤组织分化阶段和刚分化 4 周小苗的筛选浓度。结果发现，不同基因型甘蔗的愈伤组织和刚分化小苗对灭草烟的耐受浓度均有差异。综合试验结果，适合灭草烟在新台糖 22 和粤糖 93-159 愈伤组织分化阶段的筛选浓度为 0.3 滋mol/L；适合灭草烟对新台糖 22 刚分化 4 周小苗的筛选浓度为 0.5 滋mol/L，适合灭草烟对粤糖 93-159 刚分化 4 周小苗的筛选浓度为 1.0 滋mol/L。

Abstract: Strain LYM3, isolated from Rauvolfia vomitoria Afzel, had higher antagonistic effect against Colletotrichum musae, and its antagonistic activity was 64% in plate. According to its morphological characteristics, and 16S rDNA sequence analysis, the strain LYM3 was identified as Bacillus subtilis. Using single-factor method袁 the fermentation conditions of its antibacterial substance production were optimized. The optimal medium contained 2% soluble starch, 1% tryptone, 0.8% yeast extract, 1% NaCl, and initial pH was 6. After 60 h culture at 28益, 180 r/min, the antibacterial substance had higher yield, and the diameter of inhibition zone was up to 3.42 cm