基于形态与分子数据鉴定苹果异胫小卷蛾

Identification of false codling moth, Thaumatotibia leucotreta (Lepidoptera, Tortricidae) based on morphological and molecular data

摘要: 采用 DNA 条形码技术，对珠海出入境检验检疫局从南非进口的鲜橙和鲜葡萄中截获的 3 头卷蛾幼虫进行分子鉴定，采用通用引物 LCO1490/HCO2198 对其线粒体 COI 基因的部分序列进行 PCR 扩增和测序，并与 GenBank 数据库和 DNA 条形码数据库 BOLDSYSTEMS v3 中的已知序列比对，结果与 GenBank 中 20 条苹果异胫小卷蛾的序列同源性达 99%以上，与 BOLDSYSTEMS v3 中 46 条苹果异胫小卷蛾的序列同源性达 99%。结合幼虫的形态特征，综合判定该幼虫为苹果异胫小卷蛾 Thaumatotibia leucotreta。

Abstract: The technique of DNA barcoding was applied to identify three tortricid larvae intercepted by ZhuhaiEntry -Exit Inspection and Quarantine Bureau in fresh orange and grape from South Africa. Using the primerLCO1490/HCO2198, the partial mitochondrial DNA of COI gene was sequenced and blasted with released data inGenBank and BOLDSYSTEMS v3 database, the results showed that the sequences of intercepted specimens weremore than 99% indentified with 20 sequences of Thaumatotibia leucotreta in GenBank and 46 sequences of T.leucotreta in BOLDSYSTEMS v3. The larvae characters were also described in this paper. According to morphologicalcharacters and sequence, the larvae was identified as false codling moth T. leucotreta.