切花文心兰花梗芽组培快繁技术研究

Tissue culture and rapid propagation for Oncidium inflorescence shoots

摘要: 以切花文心兰南茜 Oncidium Gower Ramsey 为材料，对以花梗芽为外植体的切花文心兰组培快繁技术进行研究。结果表明，以成熟度 2（外形呈笋状，花苞和分叉始露出花梗苞片，高约 70 cm）和中部节位的花梗上的芽为最适宜外植体；用 0.1%升汞消毒处理花梗芽的适宜时间为 12 min；1/2MS+6-BA 2.0 mg/L+TDZ 1.0mg/L+NAA 0.2 mg/L 为花梗芽最佳初代诱导培养基；继代培养以 6-BA 3.0 mg/L+NAA 0.3 mg/L 为最佳激素组合；以温度 25益和光照强度 2 500 lx（光照时间 10~12 h/d）的培养条件最为理想。

Abstract: The technology of tissue culture and rapid propagation for Oncidium inflorescence shoots was studiedusing the cut -flowers of Oncidium Gower Ramsey as test material. The results showed that the shoots oninflorescence at maturity level 2 (bamboo shaped appearance, bud and bifurcation pedicel bracts, about 70 cm) andthose in the central section of inflorescence were best suitable as explants. The suitable disinfection time ofinflorescence shoots with 0.1% mercuric chloride solution was 12 min; 1/2MS+6-BA 2.0 mg/L+TDZ 1.0 mg/L+NAA0.2 mg/L was the best original induction medium. Successive transfer cultrue took 6-BA 3.0 mg/L+NAA 0.3 mg/L asthe best hormone combinations and good ideal culture conditions were at 25益and light intensity of 2 500lx (lighttime 10-12 h/d).