Abstract: Enrichment culture method was adopted to separate and purify 6 bacteria which had degradative capability. Two strains H3 and H6 with higher degradative capability were screened by studying their degradation ability. 16S rRNA gene clone method was adopted to identify their gene sequences and a phylogeny tree was constructed after a comparative analysis on similarity with the existing bacterias 16S rRNA gene sequences in the National Center for Biotechnology Information. Then, combined with their morphological, physiological and biochemical characteristics, this study determined their biological status. The results showed that 6 bacteria had certain ability to degrade pollutants, and the removal efficiencies of CODcr were better than those of TP and NH3-N; H3爷s removal rates of CODcr, TP, NH3-N were 50.2%, 26.83% and 44.89%, H6s removal rates of CODcr, TP, NH3- N were 52.19%, 25.16% and 46.79%, their degradation abilities were more prominent. H3 and Pseudomonas fluorescent (NR_113647.1) had very close affinity, their homology could up to 99%; the homology of H6 and Bacillus amyloliquefaciens (NR_075005.1) was 99%. The P. fluorescent and B. amyloliquefaciens isolated from Yellow River Wetland have purifying effect on domestic sewage, and their security is guaranteed, they can be applied to ecological restoration of lake wetlands and sewage treatment