Abstract: With the rapid growth of agricultural products logistics between Mainland and Taiwan at post-ECFA era, the games on agricultural products logistics cooperation of enterprises between Mainland and Taiwan based on the cold chain cost were increasingly outstanding. The dynamic evolutionary games were applied to build the game model of enterprises between Mainland and Taiwan, as well as the stable state and influence factors of monitoring on the cold chain cost between enterprises were analyzed. The results showed that the cooperation games between Mainland and Taiwan cold chain enterprises would be brought when the cold chain cost of Mainland enterprise was lower than the sum of punishment cost without implementing the cold chain standard and credit loss as well as the monitoring cost of Taiwan enterprise was lower than the sum of punishment cost without implementing the cold chain standard for Mainland enterprise and potential damage. The games would tend to a stable point with a fixed monitoring and implementing status when the Mainland and Taiwan enterprises were both adopting adjust strategies according to each others strategies.