Abstract: In order to achieve the efficient use of earthworm manure , by protected field trials, the impacts of different fertilization treatments including pig manure , earthworm manure , chemical fertilizers and no fertilizer on the effectiveness of soil trace elements like Fe, Mn, Cu and Zn were studied. The results showed that EM treatment reduced Mn content in soil significantly. PM treatment reduced available iron content in soil significantly. CF treatment reduced soil pH, but enhanced content of available iron, available Mn, available Cu and available Zn. The contents of soil available Mn and available Zn were negatively correlated with soil pH, but negative correlation between soil pH and the contents of available Fe and available Cu was not significant. The content of soil available Zn were positively correlated to soil organic matter, but no significant correlation existed between organic matter and other trace elements. It concluded that soil pH was the dominant factor affecting the availability of soil Mn and Zn.