Abstract: Under low temperature stress(5, 0, -5, -10) on 6 olive varieties, the results indicated that the leaf relative conductivity increased as temperature decreasing, presenting Stype curve change. The trend of leaf damage rate was consistent with the relative electric conductivity. The leaf damage rate and electric relative conductivity were different significantly in 6 varieties of olive leaves at the same treatment temperature, indirectly reflected the cold tolerance of different varieties of olive. The semilethal temperature (LT50) was conducted by using conductance method with Logistic Equation. The activity of SOD, POD and CAT generally increased first and then decreased with the temperature decreasing, the enzyme activity of Picholine was significantly higher than that of other varieties. The cold tolerance was different significantly in 6 cultivars, the hardiness of the tested cultivars was listed as Picholine﹥Pendolino﹥Leccino﹥Ascolana Tenera﹥Ezhi 8﹥Frantonio.