抗氧化酶活性作为植物抗盐性评价指标的有效性

    Effectiveness of antioxidant enzymes as evaluation index for salt tolerance of plants

    • 摘要: 以5 个抗盐性不同的树种河南白榆、新疆枸杞\南蛇藤、小叶丁香\樟子松的1 年生实生苗为试验材料,通过测定盐胁迫下5 个树种叶片丙二醛含量和超氧化物歧化酶(SOD);过氧化氢酶(CAT);过氧化物酶(POD);抗坏血酸氧化酶(APX)等抗氧化酶活性的变化,研究抗氧化酶活性作为植物抗盐性评价指标的有效性.结果表明院盐胁迫下5 个树种叶片丙二醛含量显著增加,丁香和樟子松增加幅度明显大于枸杞和白榆。SOD\CAT 可以作为不同树种抗盐性评价标准,其中SOD、CAT 更为有效。.胁迫时期抗盐性较强的枸杞和白榆SOD 活性随胁迫强度的增加逐渐升高,而抗盐性较弱的丁香和樟子松SOD 活性却不断下降;长期胁迫下抗盐性较强的枸杞和白榆CAT 活性随胁迫强度的增加逐渐升高,而抗性相对较弱的丁香和樟子松CAT 活性轻度胁迫下升高,较重胁迫下却降低.POD 和APX不是评价不同树种抗盐性的可靠指标.POD 活性增加是一种胁迫症状,而不是一种抗性表现。随胁迫强度的增加,抗盐碱性较弱的丁香和樟子松POD 活性增加,而抗盐碱性较强的枸杞和白榆POD 活性却降低.

       

      Abstract: The effectiveness of activitiy of antioxidant enzymes as evaluation index for salt tolerance were investigated in five trees differing in tolerance to salt stress by determining the malondialdehyde (MDA) content and the activities of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX). The results showed that MDA contents of the five trees increased significantly under salt stress. The increasing scales of MDA contents of Syringa microphylla and Pinus sylvestris var. mongolica were larger than that of Ulmus pumila and Lycium dasystemum. The SOD and CAT could serve as reliable indicators for appraising salt tolerance of woody plants, and SOD and CAT were more effective. With the increase of stress intensity, the SOD activities of U. pumila and L. dasystemum increased, while those of S. microphylla and P. sylvestris var. mongolica decreased obviously. POD and APX were not reliable selction criteria. Increase in POD activity did not seem to be a tolerance component, but a stress symptom.