Abstract: The experiment studied the effect of different concentration of aluminum treatment on seed germination of Stylosanthes, by taking 20 Stylosanthes as the materials. The results showed that there were significant differences in the germination rate, germination potential, germination index and root tolerance index under different concentration of Al. For some Stylosanthes, these indexes showed downward trend with the increase of aluminum concentration, while for others, the seed germination was promoted by high aluminum stress.