Abstract: AP2/ERF is a large family of transcription factors in plants, which has great effect on plant growth and physiology. The full-length coding sequence of transcription factor SHINE (SHN) from Populus tomentosa was cloned. Real-time PCR results showed that PtoSHN expressed in various organs in poplar, especially in the stem. Phylogenetic tree proved that PtoSHN shared a high similarity with AtSHN2 and AtSHN3 from Arabidopsis thaliana. The AtSHN2 has proved to be involved in plant secondary wall synthesis regulation. PtoSHN located in the nucleus, which was predicted by bioinformational software CELLO v.2.5, and then testified by subcellular localization. Therefore, we successfully cloned SHN from P. tomentosa and speculated that SHN gene may play a key role in regulating trees爷secondary growth.