醇提七星剑花多糖工艺研究及其DPPH自由基清除能力评价

Study on bath extraction technology of polysaccharide from Hylocereus undatus（Haw.）Britt. et. Rose by ethanol water and evalution on DPPH free radical scavenging ablity of the polysaccharide

摘要: 以肇庆七星剑花为材料，通过单因素试验和L9（3^4）正交试验获得乙醇水浴提取多糖的最佳工艺条件为：提取时间20 min、提取3 次、料液比1：60（g/mL）、乙醇体积分数80%；运用苯酚-硫酸比色法与蒽酮-硫酸比色法测定剑花多糖含量分别为19.80%与20.63%，后者可作剑花多糖的常用测定方法。系统比较了剑花全花、花蕊、花瓣和花萼等不同部位多糖含量的差异，花蕊多糖含量最高，达20.65%；同时应用DPPH 法评价该多糖的抗氧化活性（EC50）为5.391 μg/mL。

Abstract: The conditions for ethanol water bath extraction of polysaccharide from Hylocereus undatus（Haw.）Britt. et.Rose were optimized by single facfor test and L9 （3^4） orthogonal array design methods. A material-to-liquid ratio of 1：60(g/mL) of 80% ethanol for 20 min extractions performed thrice proved to be the optimal. Using phenol sulfuric acid method and anthrone sulfuric acid method for determination of H. undatus polysaccharide content, the contents of polysaccharide were 19.80% and 20.63%. By comparing the content of polysaccharide from stamens, flower, petal and calyx of H. undatus, we found that flower polysaccharide content was the highest, up to 20.65%. And evaluation of the antioxidant activity of the polysaccharide by DPPH method, EC50 was 5.391 μg/mL.