Abstract: On the basis of single-factor tests, we optimized process conditions of water celery natural fermentation from Guizhou. The effects of temperatures, salt concentration and spice content on the total acid content and nitrite peak were studied by using response surface analysis. The results showed the optimum conditions were as follows, temperatu36.11℃, salt concentration 4.05% and spice content 4%. Under these conditions, the total acid content was 0.715 g/100g and the nitrite peak was 4.09 mg/kg.