Abstract: Using the method of Real Time-PCR, this study investigated the tissue expression pattern of pyruvate dehydrogenase kinase 4 (PDK4) gene in broilers, the tissue expression tendency in different growth stage of broilers and the differential tissue expression in broilers in different growth rate. The results showed that: (1) Chicken PDK4 gene was predominantly expressed in muscle tissues including breast muscle, leg muscle and heart. (2) The expression pattern of PDK4 gene in muscle tissues at different growth stages indicated that it was at a low level at 12 embryo age, and had thehighest primary body expression at 1 day, then it declined gradually. (3) In pituitary, breast muscle and heart, the expressions of PDK4 gene in Xinghua Chicken were higher than those in White Recessive Rock, which was reversed in leg muscle; in breast muscle, the expression of PDK4 gene in the light weight group was higher than that in high weight group,which was reversed in leg muscle.