Abstract: The 42 plots under semi-humid evergreen broad-leaved forest were set up in central Yunnan and the macrofungi of Russulaceae was researched. 713 specimens were collected, after identification, they belonged to 71 species (49 species belonged to Russula and 22 species belonged to Lactarius). Among them, there were 49 edible species, 17 medicinal species and 10 poisonous species. Then the data were analysed respectively at different month, age-class of forest, temperature, precipitation, hours of illumination, altitude, humus thickness, slope and aspect. The results displayed that the macrofungal distribution of Russulaceae correlated closely with variation of month, age-class of forest, temperature, precipitation, hours of illumination. Moreover, the influences of humus thickness, slope and aspect were not significant for the macrofungal distribution of Russulaceae.