Abstract: Researching the effect of chemical control of Stellera chamaejasme L. on soil chemical properties in grassland in Qinghai Lake area is to review the advantages and disadvantages of chemical control of S. chamaejasme L.Collecting soil samples from chemical control of S. chamaejasme L. and non-chemical control of S. chamaejasme L. in grassland respectively, soil chemical properties were analyzed. Results showed that chemical control of S. chamaejasme L.obviously inhibited the soil pH, available nitrogen and available phosphorus, indicating that S. chamaejasme L. was benefit to improve and sustain soil fertility. The chemical control of S. chamaejasme L. had no benefit to assemble the available nitrogen and phosphorus, but it had benefit to increase the total K and organics of the soil in following year. However, the chemical control of S. chamaejasme L. had no signifcant effect on the soil chemical properties in the 0~30 cm soil layer of the grassland with the soil depth.