基于线粒体COⅡ基因的3 种土白蚁系统发育分析

Phylogenetic analysis of three Odontotermes holmgern based on mitochondrial COn sequences

摘要: 对海南的3 种土白蚁15 个不同地理种群的线粒体CO域基因进行了克隆鉴定和序列分析，并结合GenBank 中的数据，构建进化树进行遗传变异分析。结果表明，3 种土白蚁种间同源性为92.5%~95.1%，遗传分歧为5.1%~8.1%；黑翅土白蚁和海南土白蚁CO域基因不同种群之间的同源性分别为97%~99.9%和98.4%~100%，遗传分歧分别为0.1%~3.0%和0%~1.6%。崖城的一个未知种群Odontotermes sp. 与来自日本的O. javanicus 同源性最高(95.4%）。土白蚁CO域基因序列中AT 含量为61.4%，高于CG 含量，碱基变异的主要形式为颠换。

Abstract: Phylogenetic and sequence analysis of Cytochrome Oxidase Subunit II gene (CO gene) were conducted to elucidate the relationships among 15 populations of 3 species Odontotermes from Hainan province. The results showed that the sequence identification of inter-species of Odontotermes ranged from 92.5% to 95.1%, and divergence ranged from 5.1% to 8.1%; while different populations of O. formosanus and O. hainanensis ranged from 97% to 99.9% and 98.4% to 100%, and divergence ranged from 0.1% to 3.0% and 0% to 1.6%. The sequence of Odontotermes sp. Yach shared 95.4% identification and closely genetic relationship with O. javanicus, a species from Japan. CO gene of genus Odontotermes showed high AT composition, which reached to 61.4%, and the main base variation was transversion .