贵州本地山羊POLRMT 基因启动子区SNP 的生物信息学分析

Bioinformatics analysis on the SNP in promoter region of POLRMT gene in Guizhou local goat

摘要: 为给贵州本地山羊肉质品质选育工作提供更好的科学依据，以贵州白山羊、贵州黑山羊及黔北麻羊为试验对象，探究肉质相关基因POLRMT 启动子区SNP 位点对肉质品质的影响。通过构建DNA 池筛选出SNP 位点，并采用多种生物信息学软件预测核心启动子范围、CpG 岛及转录因子。结果表明，POLRMT 基因启动子区存在2 个SNP 位点，分别为T-81A、T-289C。POLRMT 基因核心启动子范围发生改变，SNP 位点导致部分转录因子结合位点消失，MethPrimer预测到CpG 岛增加1 个。

Abstract: In order to provide the basic information and scientific proof for better heredity selection of Guizhou Local goats, three goat breeds (Guizhou Write Goat, Guizhou Black Goat and Qianbei Ma Goat) were selected as the experimental subjects for exploring the influence between SNPs which located in the promoter region of POLRMT gene and meat quality. SNPs were screened by constructing the DNA pool, and variety bioinformatics softwares were used to predict the core region of the promoter, CpG island and transfer factors. The results showed that two SNPs (T-81A, T-289C) were found in POLRMT gene. Because of the two SNPs, the range of POLRMT gene core promoter changed, transcription factors disappeared, meanwhile, one CpG island increased.