Abstract: The effects of different kinds and concentrations of hormones on embryonic callus induction and plantlet regeneration were studied by using the aseptic seedling爷s cotyledon from the seeds of Siraitia grosvenorii as explants for setting up the effective regeneration system. The results indicated that the callus induction rate reached 100% under the different proportion of 6-BA and 2, 4-D or 6-BA and NAA. NAA was more suitable than 2, 4-D for embryonic callus induction. The optimum medium for embryonic callus induction was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L, and the induction rate reached 76%. The friable, granular, light yellow or yellow embryonic callus was selected. The optimum concentration of TDZ for differentiation of adventitious buds was 2.0 mg/L, with the differentiation rate 83.3% . Histo -cytological observation showed that regenerated plants were produced via organogenesis. The vascular tissue was formed firstly in inner layer of embryonic callus when the surface of embryonic callus turned green. Many vessels were observed in the green nodule. With further development, multiple meristamic centers could be found in area of near surface and they developed adventitious buds finally.