Abstract: Nowadays, the shortage and uneven distribution of water resources hindereds the agricultural development. And the heavy metal pollution of soil seriously threats the human health through the food chain. Tobacco is thermophilic crop and the chilling injury damages its quality. In this article, authors summarized the changes of physiological and biochemical indicators caused by the water, heavy metal and low temperature stress in tobacco. It could provid theoretical basis for improving stress-resistance measures. The research trend of tobacco under stress was forecasted on the future as well.