源宝占抗稻瘟病遗传分析及基因定位

    Genetic analysis and gene mapping of rice blast resistance in an indica rice Yuanbaozhan

    • 摘要: 稻瘟病是水稻三大病害之一,严重威胁着粮食安全。解决这一问题最安全、有效的措施是选育、推广抗病品种。源宝占是一份新育水稻材料,经鉴定,源宝占对来自广东各稻作区的25 个菌株中23 个表现为抗性,抗频达92%,为广谱抗性材料。以源宝占与粤香占进行杂交,构建F1、F2群体,选用GD00-193a 菌株对亲本及世代群体进行接种鉴定,结果表明F2代单株抗感分离比符合3颐1,这说明源宝占对菌株GD00-193a 的抗性是由一对显性基因或一个主效QTL 控制。利用群体分离分析法(BSA)结合隐性群体分析法(RCA)将此基因定位于标记RM136 与RM549 之间。

       

      Abstract: Rice blast, caused by fungus Magnaporthe grisea, is one of the most serious diseases of rice and has become a serious threat to food security. Yuanbaozhan is a new rice material. Improving the rice resistance to rice blast through genetic improvement methods is the most effective and safe way in the present. Yuanbaozhan has an broad -spectrum resistance to rice blast pathogen, and it has high resistance to 23 strains among 25 blast strains collected from different regions in Guangdong province. Its resistance frequency is 92%. In this study, we made a combination to establish genetic population F1, F2 among which population is from the crosses between Yuanbaozhan and Yuexiangzhan. Evaluation was conducted with Magnaprthe oryzae isolate GD00-193a, the results showed that the resistant and susceptible plants in F2 population fitted the ratio of 3:1, indicating that the resistance of Yuanbaozhan to the rice blast was controlled by a dominant gene or a QTL locus. With the bulked-extrems and recessive-class approach analysis, the rice blast resistance gene was mapped at the genome region between RM136 and RM549.