Abstract: The sur vi valr at e and gr owh st at us ofi n vi t r o pl ant l et s ofSi r ai t i a gr osvenor i iwi t h r oot s wer e i nvest gat ed under di f f er entcondi t i ons,i ncl udi ng medi um,pl ant l etqual i t y,humi di t y,t emper at ur e and f er t i l i zat i on concent r at i on.The r esul t sshowed t hatmedi um,pl ant l etqual i t y,humi di t y and t emper at ur e wer e mai n f act or st o ensur e successf ul t r anspl ant i on.M eanwhi l e,t he combi nat i on ofpond si l t ,nur ser y soi l ,ver mi cul i t e wast he opt i malmedi um f ort he i n vi t r o pl ant l ett r anspl ant at i on,t he sur vi valr at e oft he vi t r o pl ant l et sr eached 93. 56% and t he pl ant sgr ew vi gor ousl y.The sui t abl e envi r onmentcondi t i onswer e humi di t y 80% -90% and 25-30益.The bestf er t i l i zat i on concent r at i on was0. 5% ,undert hi s condi t i on,t he t r anspl ant sgr ew f astand no f er t i l i zert oxi ci t y wasobs er ved,i twasmostsui t abl e f ort r anspl ant i ng i n f i el d.