Abstract: Housefly (Musca domestica) maggots with different instars and under different culture conditions were stimulated at different temperature, supplemented by a certain time of recovery training, and then the antibacterial activity was measured. The results showed that the appropriate degree of heat shock could significantly improve the antibacterial ability, and had different effects on the antibacterial ability by different temperatures, different hot shock time, different recovery time after heat shock, and different instar maggot. The optimum condition was 10 min at 65益heat shock treatment, and then recovery for 3 h at 22益. The antibacterial activity increased by about 53 times under the condition compared to the control. Multiple batch repeatable experiments for different materials showed that the treatment had good reproducibility and high stability for the optimum condition. Different handling capacity test showed that heat treatment for large quantities of material would cause the efficiency and stability for induction effect of antibacterial activity getting lower; however, appropriate packaging for bulk material could effectively solve this problem.