Abstract: The acute toxicity of glutaraldehyde, benzalkonium bromide, iodine methionine and 1,3-Dibromo-5,5- Dimethylhydantoin (DBDMH) to juvenile Babylonia areolata was studied. The results showed that the order of toxicity was benzalkonium bromide>DBDMH>iodine methionine>glutaraldehyde, and the median lethal concentrations (LC50) of above disinfectants were 15.00 mg/L, 39.95 mg/L, 60.13 mg/L, 91.88 mg/L respectively in 24 h, and the LC50 were 7.59 mg/L, 14.73 mg/L, 32.02 mg/L, 48.55 mg/L, respectively in 48 h; and their SC (safe concentration) were 0.58 mg/L, 0.60 mg/L, 2.73 mg/L, 4.07 mg/L, respectively. We can use iodine methionine to disinfect B. areolata under safe concentration, but should not use glutaraldehyde and DBDMH, and can use benzalkonium bromide carefully.