王草根际解磷菌的分离及解磷能力测定

Isolation and phosphate solubilizing capacity determination of rhizosphere phosphate-solubilizing microorganism in King grass

摘要: 选用蒙金娜有机培养基, 从王草根际分离出21 株具有溶磷能力的菌株, 利用溶磷圈法和钼锑抗比色法对其解磷能力进行测定。结果显示、21 株菌株在蒙金娜有机磷培养基上均形成明显透明圈, 其培养48 h D/d 值在1.3~7.0 之间, 72 h D/d 值在1.72~5.5 之间, 120 h D/d 值在1.33~4.67 之间；用钼锑抗比色法测定溶磷量为4.11~327.1 mg/mL。经解磷能力分析, 菌株2-6尧2-7-1尧2-9尧4-4 具有较强的解磷能力、这些菌株在后续微生物菌肥研制中具有较大潜力。

Abstract: Phosphate-solubilizing microorganisms were isolated from King grass rhizospheres using Mengjinna organic culture media. The phosphate solubilizing capacities were investigated from 41 strains based on phosphate-solubilizing circle and molybdenum blue method. The results indicated that 21 strains had ability to solubilize organic phosphate on Mengjinna organic culture medium and 48 h D/d (diameter of phosphate-dissolving zone/diameter of bacterial clone) value was from 1.3to 7.0; 72 h D/d value was from 1.72 to 5.5; 120 h D/d value was from 1.33 to 4.67. In liquid culture, the available phosphate increment was from 4.11 to 327.1 mg/mL using molybdenum blue method by inorganic phosphatesolubilizing microorganism. Strains 2-6, 2-7-1, 2-9 and 4-4 had higher ability of organic phosphate dissolving, showed great potential for agricultural use in microbiological fertilizer.