Abstract: Oil palm is one of the predominant tropical crops which can produce palm oil. Palm oil extracted from oil palm fruits, is not only a kind of edible oil, but also raw material for industriy and biodiesel. Sterilization is the first step in the extracted process of palm oil. In this research, the influence of treat temperature, holding time, fruit storage time and sterilization capacity on the oil yield was studied. The results showed that the oil palm fruits should be sterilize as soon as possible after harvest, the ratio of oil palm fruits volume accounted for the sterilize tank volume should not be more than 0.8 under 120 for 60 min, then we could get higher oil yield.