Abstract: The research used weak gluten variety Ningmai 9, middle gluten variety Yangmai 11 and strong gluten variety Zhongyou 16 as materials, each variety took four kinds of fertilizer treatments with two replications, analyed grain protein content, hardness, flour yield, gluten content, falling number, solvent retention capacity, starch pasting properties and farinograph parameters. The results showed that, compared with no fertilizer, reducing the application of fertilizer was helpful to the quality of weak gluten wheat. In the process of fertilizers application, high proportion of base fertilizer was good for the quality of weak gluten wheat, high proportion of jointing fertilizer was harmful to the quality of weak gluten wheat. Compared with no fertilizer, in the existing soil fertility conditions, improving the fertilizer application could improve the quality of middle gluten wheat. At the same time, we should reduce the proportion of base fertilizer appropriately and control the proportion of jointing fertilizer. Compared with no fertilizer, increasing fertilizer application could significantly improve the quality of strong gluten wheat, expanding the proportion of jointing fertilizer could also improve the quality of strong gluten wheat.