Abstract: The seedballs of introduced 51 Hippeastrum vittatum varieties were planted in the greenhouse without heating in winter in Guangzhou, and artificial pollination, bearing fruit, and seedling were further investigated. The survey revealed that, seeds could be obtained by self-pollination of 29 varieties or hybridization among 309 combinations. In total, 621 ripened fruits and 35 114 full seeds were harvested through self-pollination and crossbreeding. Furthermore, 18 045 seedlings were obtained by breeding in pot, with a seedlings productivity of 51.4%. In addition, we also found that genotype had great effect on the percentage of seeding and seedling.