AB-8大孔吸附树脂纯化苦槛蓝黄酮的工艺研究

Discussion on technique for purification of Myoporum bontioides A. Gray flavonoid by AB-8 macroporous resin

摘要: 苦槛蓝中含有丰富的黄酮类化合物，极具开发利用价值。通过静态及动态吸附解吸试验，研究了苦槛蓝黄酮的大孔树脂分离纯化工艺。结果表明，AB-8 型树脂对苦槛蓝黄酮有较好的吸附和分离效果，其最佳纯化条件为：上样液浓度8 mg/mL，上样量35 mg/g 树脂，pH 值4.86，用20%、40%、60%乙醇梯度洗脱，洗脱剂用量为2、2、4BV。40%洗脱液中苦槛蓝黄酮的含量达43.15%，黄酮回收率为79.00%。

Abstract: Myroporum bontioides A. Gray is abundant in flavonoids. In order to fully develop the resources of flavonoids in M. bontioides A. Gray, the purification process of M. bontioides A. Gray flavonoids was researched. Macroporous resin was chosen with adsorption and desorption experiments to optimize the purification parameters. AB-8 macroporous resin was found to have good adsorption and desorption effects. The optimal technological parameters of the process were 8 mg/mL of extractions at pH 4.86 eluted by 20%, 40%, 60% ethanol with a volume of 2, 2, 4 BV, respectively, and the loaded amount was 35 mg/g. The purity of total flavonoids increased up to 43.15% after the purification, and the recovery rate maintained 79.00%.