Abstract: Anthurium andraeanum is the second tropical pot flowers in the world, and its transgenic breeding has been shown promising application. In this study, susceptibility of A. andraeanum cv. alabama to antibiotics (hygromycin and kanamyein) was studied Results showed that kanamycin and hygromycin could cause seriously explant browning and dying, and had significant inhibitory effect on the callus induction and bud differentiation. When the leaves and petioles were used as receptors, the optimal selection pressure was 100 mg/L kanamycin or 20 mg/L hygromycin; When the calli were used as receptors, the optimal selection pressure was 150 mg/L kanamycin or 40 mg/L hygromycin; The two antibiotics also could inhibit rooting and the optimal selection pressure was 150 mg/L kanamycin or 40 mg/L hygromycin.