不同添加物及浓度对铁皮石斛组织培养的影响

Effects of different additives and concentrations on the tissue culture of Dendrobium officinale

摘要: 以无菌播种取得的铁皮石斛无菌苗作为试验材料，研究1/2 MS 培养基中添加不同浓度的添加物对铁皮石斛的增殖、生根的影响。结果表明；马铃薯汁和木瓜乳有利于诱导芽的增殖，其中又以1/2 MS+NAA 0.2 mg/L +蔗糖30 g/L+马铃薯200 g/L 培养基最有利于诱导芽的增殖；香蕉乳有利于诱导生根，其中又以1/2 MS +NAA 0.5 mg/L+蔗糖30 g/L +香蕉100 g/L 培养基最有利于诱导芽生根。

Abstract: The effects of different additives on proliferation and rooting culture of Dendrobium officinale were studied. The results showed that: potato and papaya were conductive to bud proliferation, among which the 1/2 MS+NAA 0.2 mg/L+ sucrose 30 g/L+200 potato g/L medium was the best; banana promoted the rooting induction, in which 1/2 MS+NAA 0.5 mg/L+ sucrose 30 g/L+Banana 100 g/L medium was the best to induce the rooting of the shoots.