Abstract: The compound enzymatic extraction and alcohol precipitation process of bamboo polysaccharides was investigated, antioxidant activity in vitro was determined as well. The experimental results indicated that the optimal contents of cellulase, pectinase and papain were 360, 1 080, 7 200 U per 0.3 g sample. The optimal extraction conditions of bamboo polysaccharide were extraction time 2.2 h, extraction temperature 60益and solid to liquid ratio of 1 颐35. Subsequently, the optimum conditions of alcohol precipitation process on bamboo polysaccharides were volume percent of ethanol 80%, temperature 20益, and alcohol precipitation time 4 h. Furthermore, the crude polysaccharide showed good antioxidant activities, and its scavenging activities of DPPH free radical and ABTS free radical approached 88.1% and 86.5%, respectively.