Abstract: To optimize technical system of rapid propagation of Phalaenopsis seedling, we try to find a hormone-free method. This paper studied the effects of six kinds of fungal elicitor isolated from the wild Orchids爷s roots on platelet growth of Phalaenopsis V3 seedling. 40, 60, 80 ml/L fermentation liquid was added to the plant culture media, training 90 d, we analyzed fresh weight and chlorophyll content. Results showed that compared with the control group, 13 kinds of treat- ments was found to significantly increase the fresh weight (P<0.01), and that the inducing effects were in the order of trt6 > trt13>trt16>trt18. Compared with the control group, trt9, trt10 and trt13 were found to significantly increase the chlorophyll content (P<0.01). It can be concluded that, combined with the effect on fresh weight and chlorophyll, adding 40 ml/L Y5 or 80 ml/L Y3 is suited for the Phalaenopsis V3 seedling.