Abstract: Effects of temperature and pH on activity of 3 kinds of digestive enzymes (protease, amylase and lipase) in stomach, liver and intestine of Amphiprion ocellaris juvenile were studied and compared under artificial culture. The results showed that activity of protease, amylase and lipase all could be detected in stomach, liver and intestine, the order of protease activity was stomach > intestinal > liver, the order of amylase activity was intestinal > liver > stomach, the order of lipase activity was intestinal > liver > stomach. The optimum pH values for protease, amylase and lipase were 2.2, 7.0 and 7.5 in stomach, 7.5, 6.0 and 7.0 in liver, 7.0, 7.0 and 7.5 in foregut, 7.0, 7.0 and 7.5 in midgut, 8.0, 7.0 and 7.5 in hindgut. The optimum temperature for protease in three organs was all 40, for amylase in stomach was 40, in liver was 35 and that in intestine (foregut, midgut, hindgut) was all 30. The optimum temperature for lipase in three digestive organs was all 40.