Abstract: This paper took the preliminary trial on tissue culture and rapid propagation of Grevillea lanigera. The results showed that the contamination rate of explants could be controlled below 28.1% through the method of twice disinfection using mercuric chloride (0.025% and 0.1%). According to the results, the optimum medium for bud induction and multiplication was WPM+6-BA 1.0 mg/L+IBA 0.2 mg/L+sucrose 30 g/L; the suitable subculture cycle was 20 d; and the proliferation multiples of adventitious buds reached 1.65. The optimum medium for root induction was 1/2WPM+IBA 0.6 mg/L+sucrose 15 g/L and 91.7% of rooting percentage was obtained. The plantlet survival rate could exceed 90%.