Abstract: “Ningdun”series products developed by Nanjing Agricultural University are the microbial preparations which have the capacity of controlling widely a variety of soil borne diseases and aboveground diseases, among them the microbial preparation “Ningdun No.1”is dedicated to control solanaceous fruit vegetable specially. In this study, effects of Ningdun No.1 on a range of physiological indicators of plant growth including biomass, yield, chlorophyll content, tomato under field conditions were tested by a variety of inoculation methods. Three application methods including soaking seeds, drenching soil around seeds right after sowing, and drenching the rhizosphere of transplanted seedling with the aqueous solution (as a soil drench) and three concentrations of “Ningdun No.1”(1.0：106CFU/mL, 1.0：107 CFU/mL, and 1.0伊108 CFU/mL) were employed. The result showed that both soaking seeds with “Ningdun No.1”at 1.0：107 CFU/mL and drenching rhizosphere with “Ningdun No.1”at 1.0伊106 CFU/mL and 1.0：107 CFU/mL promoted growth, increased yield. The combination of these two inoculation methods led to significant increase of growth and fruityield. It was found that the most cost-effective way was the combination of soaking seeds with “Ningdun No.1”at 1.0：107 CFU/mL and drenching rhizosphere at 1.0伊106 CFU/mL, the method was also easy for Chinese farmers to implement.