Abstract: In this study, the effect of 6-benzylaminopurine(6-BA), kinetin(KT), thidiazuron(TDZ) in medium for tuftedudinduction from apical meristem of Yuetang 03-393 was analyzed by using orthogonal experiment design. The rate both of tufted-bud and of strengthen seedling after 30 days cultivation was respectively investigated. By means of visual analysis, the results indicated that the most important factor for the tufted-bud induction was 6-BA, followed by TDZ and KT; And the optimum consistency combination of three plant regulators in tufted-bud induction was 6-BA 1.50 mg/L袁KT 0.50 mg/L，TDZ 0.03 mg/L. But there was no significant difference among these three factors by the analysis of variance. What's more, when 6-BA is in 1.00 mg/L, the strengthen seedling rate is significantly higher than the rate both in 1.50 mg/L and in 0.50 mg/L.