Abstract: The aim of this research was to diagnose herbicide phytotoxicity and compare the effects of 6 kinds of herbicides on field of spring wheat in Qinghai. At the areas with doubling dose of maximum dose level of 2, 4-D and Fluroxypyr, the herbicide phytotoxicity of wheat reached to the degree of 4, while the herbicide phytotoxicity of wheat reached under the degree of 3 at the areas with the other 4 kinds of herbicide. The results showed that the herbicide phytotoxicity of these 6 kinds of herbicide was not serious, and 6 kinds of herbicide had little effect on the yield of spring wheat in Qinghai.