Abstract: The intelligent plant tissue culturebox consists of monitoring unit including collection, transmission, processing and display modules, and control unit including analog device and control module. Monitoring unit achieves the functions including collection,transmission, processing and display of environmental information of plant in tissue culturebox. Analog device is designed by high-power LED, low temperature electric heating film and semiconductor refigeratin piece, to achieve the functions of lighting, heating and cooling.Control module is used to realize the remote control and intelligent inferior control of temperature, humidity and light. This design has broad market prospect with simple circuit, stable performance and very scalable.