Abstract: In this paper, orthogonal experimental design L9(34) was adopted to investigate the effects of planting density and application amount of N, P and K on main agronomic traits of new potato variety Bishu No.3 breeded by Bijie Institute of Agricultural Sciences, to get high yield and quality planting technigue. The results showed that the effect order of all cultivation factors on yield was N>density>P>K,the effect order of all cultivation factors on tuber number per plant was density>K>N跃P, the effect order of all cultivation factors on tuber weight per plant was density>N>P>K, and the effect order of all cultivation factors on rate of economic potato was density>K>N>P. The highest economic benefit of Bishu No.3 would be obtained under the cultivation conditions of 4 667 plants per 667 m2, 6 kg N, 3 kg P2O5 and 6 kg K2O per 667 m2。