超声波辅助提取大黄鱼卵磷脂的工艺研究

    Ultrasonic-assisted extraction of phospholipids lecithin from Pseudosciaena crocea

    • 摘要: 以大黄鱼卵为原料,乙醇为提取溶剂,采用超声波辅助提取法提取大黄鱼卵磷脂。以钼蓝比色法测定磷脂含量,分别对超声功率、乙醇含水量、超声温度、超声时间进行单因素和正交试验,并通过极差和方差分析对提取过程显著影响提取率的因素进行统计分析。结果表明,大黄鱼卵磷脂超声波辅助提取法的最佳工艺条件为:超声功率为210 W、乙醇含水量为7%、超声温度为42℃、超声时间为20 min,该工艺条件下大黄鱼鱼卵磷脂的提取率为59.37%。该提取工艺参数稳定、可靠,提取率高,是一种较理想的提取大黄鱼卵磷脂的方法。

       

      Abstract: The phospholipids from the egg of Pseudosciaena crocea were carried out by ultrasonic extraction using ethanol as solvent and the extraction yield was measured by coloration method with colorimetry of molybdenum-blue. The effects of parameters including ultrasonic-wave power, water content in ethanol, ultrasonic-wave temperature, ultrasonic-wave time on extraction yield were investigated by single factor test and orthogonal array design methods. And the notability difference was analyzed with the statistic method of range and variance. Results showed that the optimal conditions were as follows: ultrasonic-wave power was 210 W, water content in ethanol was 7%, ultrasonic-wave temperature was 42℃, ultrasonic-wave time was 20 min. The extraction yield of the phospholipids from the egg of P. crocea was reached to 59.37%. The optimized ultrasonic extraction process was efficient, reliable, stable and it was a good method to get the phospholipids from the egg of P. crocea.

       

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