Abstract: In order to more fully understand the decomposition of glomalin-related soil protein (GRSP) and its coorelations with soil characteristics, the study was carried out by a in-situ field experiment and Braford analysis method. The results showed that, during the whole cultivating period, the loss mass of T-GRSP in the two groups (pakchoi21.1%,carrot13.1%) significantly was higher than that of EE- GRSP (pakchoi11.9%,carrot5.3%); and the loss mass of GRSP (T-GRSP and EE-GRSP) in the pakchoi group was significantly higher than that in the carrot group; the change T-GRSP content in soil was significantly positively coorelation with that of EE-GRSP. Both T-GRSP and EE-GRSP changes in soil were significantly positive correlation with soil carbohydrates (hot water extraction and hydrolysis states) content and ß-glycosidase enzyme activity changes, while presented a significantly negative correlation with soil proteinase activity and microbe biomass carbon change.