Abstract: The HbMT2 was constructed into pCAMBIA1304 expression vector, and transformed into tobacco (Nicotiana tabacum) mediated by Agrobacterium, Hygromycin B (Hyg) resistant regenerated plantlets of tobacco were selected. PCR detection indicated that all the Hyg resistant tobacco plants contained the alien HbMT2 gene. Resistance analysis tests showed that the tolerance of transgenic plants to hydrogen peroxide and ultraviolet irradiation was significantly increased, and the chlorophyll content and the activity of peroxidase and catalase were significantly higher in trangenic plants than the controls in heavy-metal stress condition.