Abstract: In order to develop an efficient and reliable tissue culture system for warm-season forage grass Hemarthria compressa, an efficient plant regeneration system via callus induction was established using immature inflorescence as the explants. The frequency of callus induction reached 100.00% in the callus induction medium (MS) with 7 mg/L 2,4-D by taking the lower immature inflorescence.The highest embryonic callus formation reached 89.44% in the subculture medium (MS) with 1 mg/L 2,4-D. The frequency of green plant differentiation and rooting reached 100.00% of the callus after subculture on MS with 1 mg/L 2,4-D, the average number of green buds and toots significantly more than other treatments. The results would be useful for development of transgenic H. compressa plants.