产氨短杆菌的诱变以及TMTD抗性突变株发酵培养基条件的优化

The mutant of Brevibacterium ammoniagenes and the optimization of culture medium for a TMTD

摘要: 通过对产氨短杆菌进行微波诱变，筛选TMTD（N,N-四甲基二硫双硫羰胺）抗性突变株以期获得辅酶A的高产菌株，并对其中菌株A3进行了培养基的初步优化。获得的优化培养基配方为：葡萄糖1.5%，蛋白胨1.0%，酵母膏0.5%，硫酸镁1%，磷酸氢二钾1%，磷酸二氢钾1%；接种量为15%。在此优化条件下，菌株的OD600可达5.12。

Abstract: TMTD resistant strains were screened for Coenzyme A high-producing strain by inducing revibacterium ammoniagenes with microwave irradiation. Subsequently, the medium of a TMTD resistant strain named A3 was optimized. The optimization medium was as follows: glucose 1.5%, peptone 1.0%, yeast extract 0.5%, magnesium sulfate 1%, dipotassium hydrogen phosphate l%, potassium dihydrogen phosphate l%; and inoculation amount was 15%. Under the optimum conditions, the average OD600 of strain A3 could reached 5.12.