Abstract: A HPLC-MS/MS method was developed for determination of 4 nitrofuran metabolite residues in liver. The sample was extracted with ethyl acetate and purified by Oasis HLB solid -phase extraction column, then detected by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Acetonitrile and formic acid-ammonium acetate buffer were used as mobile phase by gradient elution. The analysis of target compounds was carried out by HPLC-MS/MS under positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) mode. The calibration curves for 4 nitrofuran metabolite residues all had good linear relationships with correlation coefficients not less than 0.9906. The spiked recoveries were ranged from 91.0% to 101.5% with relative standard deviations less than 8%. It was an effective method which fit for the nitrofuran metabolite residues analysis and has high accuracy, precision and sensitivity.