Abstract: According to published duck plague virus (DPV)gI gene sequences, a pair of specific primers was designed and synthesized. The genomic DNA of DPV Henan strain, which was isolated from Henan, was used as template.The PCR product cloned into the PGEM-T vector. The gene was sequenced and a complete open reading frame was found by bioinformatics tools of ORF Finder and Blastn. Bioinformatics analysis showed that the ORF of 1 089 bp encoding gI gene and encoding 362 amino acids was obtained. The protein molecular weight was 39.7 ku,and its isoelectric point PI was 6.060.