绿茶毛尖中有机酸的高效液相色谱分析

Analysis of organic acids in Maojian green tea by high performance liquid chromatography

摘要: 建立了测定绿茶毛尖中有机酸的高效液相色谱方法。应用反相C18色谱柱，0.05 mol/L KH2PO4（pH=2.7）为流动相，流速为0.6 mL/min，检测波长为210 nm，柱温为30℃，使绿茶中7 种有机酸得到很好地分离。各有机酸在线性范围内呈良好的线性关系，回收率在95.6%~102.5%之间。该方法操作简单、快速、准确度高，适用于绿茶中有机酸含量的测定。

Abstract: Detectionation of organic acids in Maojian green tea by HPLC was studied. A reversed-phase C18 column was used with the mobile phase of 0.05 mol/L KH2PO4 （pH=2.7）at 0.6 mL/min and detection wavelength at 210 nm and column temperature of 30℃，it can be seperated well for the seven organic acids in MaoJian green tea. The organic acids had good linear in the linear range with recoveries between 95.6%~102.5%. The method was simple，rapid and with high accuracy which was suitable for determination of organic acids in green tea.