Abstract: The contents of total volatile acids, semi-volatile acids (higher fatty acids) and non-volatile acids in 120 flue-cured tobacco leaves grown in domestic 5 provinces in 2009 was analyzed synthetically. Thereinto, the contents of total volatile acid in tobacco leaves of these main areas from high to low were Guangxi>Hunan>Yunnan>Hubei>Guizhou, the semi-volatile acids were Yunnan>Hunan>Guizhou>Hubei>Guangxi, the non-volatile acids were Guangxi>Hubei>Hunan>Guizhou>Yunnan. The contents of total volatile acids and semi-volatile acids in middle leaves was higher than upper leaves, there is no regular changes of non-volatile acids contents and leaf position.