超高压联合护色剂抑制龙眼果肉褐变的工艺优化

    Optimization of Ultra-high Pressure Combined with Color-protecting Agent for Inhibiting Browning of Longan Pulp

    • 摘要:
      目的 针对龙眼果肉在干制加工过程中易发生酶促褐变,导致产品色泽劣变与营养品质下降的问题,建立一种高效、绿色的龙眼果肉护色技术,为龙眼干制加工提供技术支撑。
      方法 比较不同物理处理及常用护色剂对龙眼果肉褐变的抑制效果,筛选适宜的物理处理方式和护色剂组合。在此基础上,以多酚氧化酶(Polyphenol oxidase,PPO)和过氧化物酶(Peroxidase,POD)的相对残余活性及产品褐变度为主要评价指标,采用单因素试验和正交试验〔L9(34)〕优化超高压联合护色工艺参数;并将经优化工艺处理的样品进行热风干燥,测定其色泽参数、PPO和POD相对残余活性、褐变度、游离态与结合态酚类含量及抗氧化活性。
      结果 筛选结果表明,超高压处理在物理护色方法中表现较优,D- 异抗坏血酸和谷胱甘肽为较适宜的护色剂组合。优化得到的最佳工艺为:龙眼果肉经0.5% D- 异抗坏血酸与0.4%谷胱甘肽溶液浸泡后真空包装,于500 MPa压力下处理25 min。该条件下,与未处理组相比,PPO和POD相对残余活性分别显著降低至28.72%和9.34%(P < 0.05),褐变度显著降低45.71%。进一步分析发现,经联合护色处理,龙眼果干游离酚、结合酚和总酚含量均显著提高,其中游离态和结合态酚类含量分别较未处理组提高18.28%和137.82%,铁离子还原抗氧化能力(FRAP)和1, 1-二苯基-2-苦基肼基(DPPH)自由基清除能力分别提升至未处理组的5.14倍和1.29倍。
      结论 超高压联合护色剂工艺可有效抑制龙眼果肉褐变,并增强其FRAP和DPPH自由基清除能力,为龙眼干制加工中的护色处理提供参考。

       

      Abstract:
      Objective To address the problems of enzymatic browning prone to occur in longan pulp during drying processing, which causes deteriorated color and reduced nutritional quality of finished products, this study aims to develop an efficient and green color-protection technology for longan pulp and provide technical support for industrial drying of longan.
      Method The inhibitory effects of various physical treatments and common color protectants on the browning of longan pulp were compared to screen appropriate physical treatment modes and color protectant combinations. On this basis, taking the relative residual activities of polyphenol oxidase (PPO) and peroxidase (POD) as well as the browning degree of products as primary evaluation indices, single-factor experiments and an orthogonal array design L9(34) were adopted to optimize the process parameters of ultra-high pressure combined with color protection treatment. Samples treated with the optimized process were further subjected to hot-air drying, followed by determinations of color parameters, PPO and POD relative residual activities, browning degree, free and bound phenolic contents, and antioxidant capacities.
      Result Screening results demonstrated that ultra-high pressure treatment exhibited superior color-preserving performance among all tested physical methods, while D-isoascorbic acid and glutathione constituted the optimal color protectant combination. The optimized integrated process was determined as follows: longan pulp was soaked in a mixed solution containing 0.5% D-isoascorbic acid and 0.4% glutathione, vacuum-packed, and then treated under 500 MPa for 25 min. Under these conditions, the relative residual activities of PPO and POD were significantly reduced to 28.72% and 9.34%, respectively (P < 0.05), and the browning degree decreased by 45.71% compared with the untreated control group. Further analyses revealed that the combined color protection treatment significantly elevated the contents of free phenols, bound phenols and total phenols in dried longan pulp. Specifically, free and bound phenols contents increased by 18.28% and 137.82% relative to the untreated group, respectively. The ferric reducing antioxidant power (FRAP) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity of treated samples were increased 5.14 times and 1.29 times of the untreated group.
      Conclusion The integrated process of ultra-high pressure combined with color protectants effectively alleviates browning of longan and enhances its FRAP and DPPH radical scavenging capacity, which provides a technical reference for color protection during the drying processing of longan.